fulltext.study @t Gmail

Determination of proteins using fluorescence enhancement of Tb3+–benzoylacetone–sodium dodecyl benzene sulfonate–protein system

Paper ID Volume ID Publish Year Pages File Format Full-Text
29114 44122 2006 6 PDF Available
Title
Determination of proteins using fluorescence enhancement of Tb3+–benzoylacetone–sodium dodecyl benzene sulfonate–protein system
Abstract

A new system for the determination of proteins has been developed in this paper. Proteins can enhance the fluorescence intensity of the Tb–benzoylacetone (BA) system in the presence of sodium dodecyl benzene sulfonate (SDBS). Under optimum conditions, the enhanced fluorescence intensity is in proportion to the concentration of proteins in the range of 1.0 × 10−8 to 6.0 × 10−6 g mL−1 for bovine serum albumin (BSA), 3.0 × 10−8 to 6.0 × 10−6 g mL−1 for egg albumin (EA) and 1.0 × 10−8 to 5.0 × 10−6 g mL−1 for human serum albumin (HSA). Their detection limits (S/N = 3) are 3.9 × 10−9, 4.0 × 10−9 and 8.5 × 10−9 g mL−1, respectively. In addition, the interaction mechanism is also investigated.

Keywords
Fluorescence enhancement; Protein; Tb3+; Benzoylacetone; SDBS
First Page Preview
Determination of proteins using fluorescence enhancement of Tb3+–benzoylacetone–sodium dodecyl benzene sulfonate–protein system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 181, Issue 1, 5 July 2006, Pages 50–55
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us