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Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy

Paper ID Volume ID Publish Year Pages File Format Full-Text
29122 44122 2006 7 PDF Available
Title
Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
Abstract

Liposomes composed of cationic lipids and neutral phospholipids have been used as vehicles for cellular delivery of DNA and drug delivery. The aim of this study is to get a better understanding of phospholipid–cationic lipid interactions that is essential for the development of this potential. We have studied the interaction between cationic lipid liposomes primarily composed of dioctadecyldimethylammonium bromide (DODAB) with two different phospholipids, dioleoyl phosphatidylethanolamine (DOPE) and soybean phosphatidylcholine (PC), as well as with cholesterol. The effect of these components on the liposome's physical properties (microviscosity and polarity) was accessed by studying, both the steady-state and time-resolved, fluorescence anisotropy of the dye Nile Red. This information combined with analysis of the steady-state emission and fluorescence lifetime of Nile Red using the different lipids and cholesterol provided information concerning the polarity and hydration level changes in these vesicle systems along with organisational information.

Keywords
Ch, cholesterol; DODAB, dioctadecyldimethylammonium bromide; DOPE, dioleoyl phosphatidylethanolamine; NR, Nile Red; PC, soybean phosphatidylcholineDODAB; DOPE; Cholesterol; PC; Nile Red; Fluorescence anisotropy
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Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 181, Issue 1, 5 July 2006, Pages 99–105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us