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10-(2-Biotinyloxyethyl)-9-acridone: A novel fluorescent label for (strept)avidin–biotin based assays

Paper ID Volume ID Publish Year Pages File Format Full-Text
29126 44122 2006 6 PDF Available
Title
10-(2-Biotinyloxyethyl)-9-acridone: A novel fluorescent label for (strept)avidin–biotin based assays
Abstract

A novel biotinylated fluorophore, 10-(2-biotinyloxyethyl)-9-acridone 3 has been synthesized and its fluorescent properties were examined in the presence and absence of avidin or streptavidin. In aqueous solutions the novel fluorophore 3, as well as its precursor 10-(2-hydroxyethyl)-9-acridone 2 exhibit intense fluorescence and can be detected down to 8.62 × 10−10 and 1.90 × 10−10 M, respectively. A short spacer was chosen in order to minimize steric repulsion between the adjacently bound to avidin or streptavidin residues of acridone. The novel biotinylated fluorophore 3 exhibits rapid, specific and stoichiometric binding to the four biotin binding sites in avidin or streptavidin tetramers, even at low concentrations (20 nM). Preliminary measurements showed that the new conjugate can be applied for the fluorimetric determination of solid-phase immobilized mouse IgG with detection limits down to 1.3 ng per assay.

Keywords
Acridone; Biotin; Avidin; Streptavidin; Fluorescence
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10-(2-Biotinyloxyethyl)-9-acridone: A novel fluorescent label for (strept)avidin–biotin based assays
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 181, Issue 1, 5 July 2006, Pages 126–131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us