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Spectroscopic and calorimetric studies on the binding of the phototoxic and cytotoxic plant alkaloid sanguinarine with double helical poly(A)

Paper ID Volume ID Publish Year Pages File Format Full-Text
29148 44127 2008 11 PDF Available
Title
Spectroscopic and calorimetric studies on the binding of the phototoxic and cytotoxic plant alkaloid sanguinarine with double helical poly(A)
Abstract

The interaction of the phototoxic and cytotoxic DNA binding plant alkaloid sanguinarine with double stranded polyadenylic acid has been investigated from multifaceted spectroscopic and calorimetric techniques. The hypochromic and bathochromic shifts in the absorption spectrum, and large enhancement of the fluorescence intensity of sanguinarine indicated strong binding of the alkaloid to the duplex poly(A). The corresponding intrinsic binding constant for the complexation, obtained from visible absorbance and fluorescence spectroscopic methods, respectively, was found to be in the order of 104 M−1. The binding was further characterized by strong energy transfer from the adenine base pairs to the alkaloid, significant polarization of the fluorescence of the alkaloid, stabilization against thermal strand separation of the duplex, and also resulting in remarkable conformational changes in poly(A) with concomitant generation of optical activity in the bound alkaloid molecules that are otherwise achiral. Isothermal titration calorimetric and differential scanning calorimetric studies revealed the binding of the alkaloid to be exothermic and enthalpy driven. All these results together with fluorescence quenching experiments advance good evidence for the intercalation of the alkaloid sanguinarine into the duplex poly(A).

Keywords
DNA, deoxyribonucleic acid; RNA, ribonucleic acid; poly(A), polyriboadenylic acid; P/D, nucleotide phosphate/alkaloid molar ratio; D/P, alkaloid/nucleotide phosphate molar ratio; CP, citrate phosphate; CD, circular dichroism; Tm, thermal melting temperatu
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Spectroscopic and calorimetric studies on the binding of the phototoxic and cytotoxic plant alkaloid sanguinarine with double helical poly(A)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 194, Issues 2–3, 20 February 2008, Pages 111–121
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us