fulltext.study @t Gmail

Highly efficient enzymatic synthesis of Z-aspartame in aqueous medium via in situ product removal

Paper ID Volume ID Publish Year Pages File Format Full-Text
2917 142 2015 5 PDF Available
Title
Highly efficient enzymatic synthesis of Z-aspartame in aqueous medium via in situ product removal
Abstract

•Z-Aspartame was highly efficient synthesized in low pH aqueous medium.•High yield of Z-APM was achieved due to “in situ product removal”.•Rationalized the inhibition by Z-l-Asp at a relatively higher concentration using molecular docking approach.

Z-Aspartame (Z-APM) was obtained using an aqueous reaction at pH 6.0 based on thermodynamic control and in situ product removal. The lower pH reaction medium led to higher solubilities of the two substrates, l-PheOMe and Z-l-Asp and dramatically lowered the solubility of the product. The higher pH stability of the protease PT121 and the mutant Y114S enabled the pH modulation of the reaction medium to shift the thermodynamic equilibrium toward product synthesis. The reaction-separation coupling provided a “driving force” for the enzymatic synthesis and resulted in high yields of 88.5% (CZ-l-Asp = 50 mM, Cl-PheOMe = 500 mM) and 82.2% (CZ-l-Asp = 100 mM, Cl-PheOMe = 500 mM), without further purification for removing protection group, of Z-aspartame. Moreover, we rationalized the inhibition by Z-l-Asp at a relatively higher concentration on the synthesis of Z-APM using a molecular docking approach.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Z-aspartame; Biocatalysis; Protease; Enzyme technology; Mass transfer; Molecular docking
First Page Preview
Highly efficient enzymatic synthesis of Z-aspartame in aqueous medium via in situ product removal
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 98, 15 June 2015, Pages 63–67
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us