fulltext.study @t Gmail

The interaction of silibinin with human serum albumin: A spectroscopic investigation

Paper ID Volume ID Publish Year Pages File Format Full-Text
29173 44127 2008 11 PDF Available
Title
The interaction of silibinin with human serum albumin: A spectroscopic investigation
Abstract

Silibinin is a well-known naturally occurring compound used for the treatment of a wide range of hepatitic diseases. In this study, we report the binding of silibinin to the carrier protein, human serum albumin (HSA) under physiological conditions. UV–Vis and fluorescence quenching methods in combination with Fourier transformed infrared (FT-IR) and circular dichroism (CD) spectroscopy have been used to study the nature and mode of binding. The binding parameters were determined from tryptophan fluorescence quenching by a Scatchard plot and the results were found to be consistent with those obtained from a modified Stern–Volmer equation. The association constant is of the order of 105. The change in enthalpy (ΔH°) and entropy (ΔS°) due to the interaction were found to be −6.76 kJ mol−1 and 73.69 J mol−1 K−1, respectively. These values suggest that apart from an initial hydrophobic association, electrostatic interactions play a decisive role during complexation of silibinin with HSA. Observations from FT-IR and CD spectra upon ligand binding indicate changes in the secondary structure of HSA. Docking studies that corroborate our experimental results reveal that the silibinin molecule lies within hydrogen bonding distance of Trp 214 and Asp 451 residues of subdomains IIa and IIIa of HSA, respectively.

Keywords
Silibinin; Human serum albumin; Fluorescence; Fourier transformed infrared; Circular dichroism; Docking
First Page Preview
The interaction of silibinin with human serum albumin: A spectroscopic investigation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 194, Issues 2–3, 20 February 2008, Pages 297–307
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us