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Coherent control improves biomedical imaging with ultrashort shaped pulses

Paper ID Volume ID Publish Year Pages File Format Full-Text
29197 44130 2006 7 PDF Available
Title
Coherent control improves biomedical imaging with ultrashort shaped pulses
Abstract

Although ultrashort pulses are advantageous for multiphoton excitation microscopy, they can be difficult to manipulate and may cause increased sample damage when applied to biological tissue. Here we present a method based on coherent control that corrects phase distortions introduced by high numerical aperture (NA) microscope objectives, thereby achieving the full potential of ultrashort pulses. A number of useful phase functions are recommended to gain selectivity that is similar to that which can be achieved by tuning a longer laser pulse; however this one involves no moving parts and maintains perfect optimization. This capability is used to demonstrate functional imaging by selective two-photon excitation of a pH-sensitive chromophore. Finally, we show that phase functions can also be introduced to minimize multiphoton excitation damage, while maintaining a high efficiency of two-photon excitation.

Keywords
Coherent control; Phase modulation; Shaped pulses; Femtosecond; Two-photon imaging; Three-photon
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 180, Issue 3, 25 June 2006, Pages 307–313
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us