fulltext.study @t Gmail

Application of a novel respirometric methodology to characterize mass transfer and activity of H2S-oxidizing biofilms in biotrickling filter beds

Paper ID Volume ID Publish Year Pages File Format Full-Text
2923 143 2015 11 PDF Available
Title
Application of a novel respirometric methodology to characterize mass transfer and activity of H2S-oxidizing biofilms in biotrickling filter beds
Abstract

•A novel respirometer design characterized the kinetics in H2S-oxidizing biofilms.•H2S biofiltration properties were evaluated from a sample of biotrickling filter bed.•Short-term respirometric assays (<20 min) were performed.•A mathematical model of the biotrickling filter bed respirometry was developed.•The non-wetted biofilm fraction contributed 65% to the overall removal of H2S.

The elimination capacity of gaseous H2S biofiltration can be limited either by mass transfer or bioreaction in the biofilm. Assessment of the biological activity of immobilized cells (biofilm) usually implies morphological and physiological changes during the adaptation of cells to respirometric devices operated as suspended cultures. In this study, respirometry of heterogeneous media is advised as a valuable technique for characterizing mass transport and biological activity of H2S-oxidizing biofilms attached on two packing materials from operative biotrickling filters. Controlled flows of liquid and H2S-containing air were recirculated through a closed heterogeneous respirometer allowing a more realistic estimation of the biofilm activity by the experimental evaluation of the oxygen uptake rate (OUR). Specific maximum OUR of 23.0 and 38.5 mmol O2 (g biomass min)−1 were obtained for Pall rings and polyurethane foam, respectively. A mathematical model for the determination of kinetic-related parameters such as the maximum H2S elimination capacity and morphological properties of biofilm (i.e., thickness and fraction of wetted area of packing bed) was developed and calibrated. With the set of parameters obtained, the external oxygen mass transport to the wetted biofilm was found to limit the global H2S biofiltration capacity, whereas the non-wetted biofilm was the dominant route for the gaseous O2 and H2S mass transfer to the biofilm. Oxygen diffusion rate was the limiting step in the case of very active biofilms.

Keywords
OUR; Hydrogen sulfide; Heterogeneous respirometry; Wetted/non-wetted biofilm; Mathematical model; Mass transfer
First Page Preview
Application of a novel respirometric methodology to characterize mass transfer and activity of H2S-oxidizing biofilms in biotrickling filter beds
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 99, 15 July 2015, Pages 24–34
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us