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Lipid production by eukaryotic microorganisms isolated from palm oil mill effluent

Paper ID Volume ID Publish Year Pages File Format Full-Text
2925 143 2015 7 PDF Available
Title
Lipid production by eukaryotic microorganisms isolated from palm oil mill effluent
Abstract

•Palm oil mill effluent (POME) contained microorganisms able to accumulate lipids.•Community composition of the organisms enriched from POME was analyzed.•Graphium penicilloides isolated from POME contained more than 20 wt% lipids.

Microbial oil production combined with wastewater management is one option for a more sustainable future. Micrographs of microbial cultures enriched from palm oil mill effluent (POME) showed lipid inclusion in the eukaryotic cells, indicating the cells can accumulate lipids. However, enriching the culture did not increase the total lipids. Therefore, eukaryotic microorganisms were isolated from POME to investigate whether these microorganisms are potential lipid producers. Four strains were isolated, and their lipid synthesis capabilities were compared with known oleaginous yeasts in a synthetic oil-free medium. Two strains (identified as Galactomyces geotrichum and Graphium penicillioides) had the potential to accumulate lipid accumulation based on the increase in triacylglycerol content. G. penicillioides was the most promising strain for lipid production as this strain accumulated more lipids than the well-known oleaginous yeast Cryptococcus curvatus (29.1 ± 3.0 wt% vs. 20.2 ± 2.9 wt%). To our knowledge, oil synthesis and accumulation by G. penicillioides have not previously been reported.

Keywords
Filamentous fungi; Lipid accumulation; Microbial growth; Palm oil mill effluent; Physiology; Yeast
First Page Preview
Lipid production by eukaryotic microorganisms isolated from palm oil mill effluent
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 99, 15 July 2015, Pages 48–54
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering