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Poly(methacrylic acid) enhances emission of dye stain extracted from bacterial spores: Fluorimetric quantitation in aqueous samples

Paper ID Volume ID Publish Year Pages File Format Full-Text
29258 44143 2007 6 PDF Available
Title
Poly(methacrylic acid) enhances emission of dye stain extracted from bacterial spores: Fluorimetric quantitation in aqueous samples
Abstract

A new fluorometric procedure is demonstrated for fast detection of dormant bacterial endospores in suspension. The method is based on quantitative uptake of the cationic dye stain malachite green (MG) by permeabilized spores. Specifically bound stain is extracted from the spores into poly(methacrylic acid) (PMAA) acidic solution. The steady-state fluorescence enhancement of MG in buffered PMAA-citrate is then employed for determination of dye uptake by spores at the nanogram scale. The assay tolerates the presence of anions, surfactants and other potentially masking species, that may be present in environmental samples. The analytical procedure is simple and allows sensitive (<105 ml−1) identification of bacterial spores in aqueous samples within 1–2 h, in presence of possible masking factors, such as salts (buffers), surfactants and neutral particles.

Keywords
Fluorescence enhancement; PMAA; Malachite green; Bacterial spore detection; Fluorescent assay
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Poly(methacrylic acid) enhances emission of dye stain extracted from bacterial spores: Fluorimetric quantitation in aqueous samples
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 189, Issues 2–3, 25 June 2007, Pages 147–152
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us