fulltext.study @t Gmail

Cell disruption of S. cerevisiae by scalable high-intensity ultrasound

Paper ID Volume ID Publish Year Pages File Format Full-Text
2929 143 2015 8 PDF Available
Title
Cell disruption of S. cerevisiae by scalable high-intensity ultrasound
Abstract

•Novel horn geometry permits industrial-scale ultrasonic lysis of yeast.•Barbell Horn Ultrasonic Technology (BHUT) disrupts S. cerevisiae cells on a large scale.•High ultrasonic amplitudes are required for efficient S. cerevisiae cell disruption.•Ultrasonic amplitude plays significant role in membrane-bound protein extraction.

Ultrasonic disruption of yeast and other microbial cell cultures is commonly used for laboratory-scale protein preparations because it is rapid, efficient and simple to use compared to such methods as high-pressure homogenization (HPH) and bead milling. Lysing by sonication is also more effective than other cell disruption methods for the recovery of periplasmic, membrane-bound, or insoluble recombinant proteins. Until recently, however, due to amplitude limitations of conventional-design pilot and industrial-size sonication equipment, ultrasonic cell disruption was only feasible on the laboratory scale. In this study, we show that Barbell Horn Ultrasonic Technology (BHUT) can be successfully used for the disruption of Sacharomyces cerevisiae (S. cerevisiae) cells on a large scale. In particular, we show that by using pilot-scale BHUT-based equipment, total protein and alkaline phosphatase can be efficiently extracted from S. cerevisiae cells, achieving about an order of magnitude productivity increase factor with respect to laboratory-scale results. Since the size of BHUT-based ultrasonic processors can be increased further, ultrasonic cell disruption now has the ability to develop into a valuable commercial-scale method, potentially superior to HPH and bead milling techniques in this area of application.

Keywords
Cell disruption; Scale-up; Yeast; Production kinetics; Sonication; Barbell Horn Ultrasonic Technology (BHUT)
First Page Preview
Cell disruption of S. cerevisiae by scalable high-intensity ultrasound
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 99, 15 July 2015, Pages 99–106
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us