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A novel peptide for efficient siRNA delivery in vitro and therapeutics in vivo

Paper ID Volume ID Publish Year Pages File Format Full-Text
293 23 2015 11 PDF Available
Title
A novel peptide for efficient siRNA delivery in vitro and therapeutics in vivo
Abstract

Small interfering RNA (siRNA) shows great therapeutic potential due to its ability to regulate gene expression in a highly selective manner. However, its application has been limited by ineffective cellular uptake of siRNAs. To achieve successful gene-silencing efficiency, a safe and effective delivery vector is generally required. In this study, we designed a series of amphipathic peptides that comprised a variant of a nuclear localization sequence, 0–6 histidine residues and an optional stearic acid group. Among these candidates, STR-HK exhibited good characteristics as a safe and efficient siRNA delivery vector, facilitating efficient siRNA delivery to mammalian cells without causing cytotoxicity. Moreover, the intratumoral injection of STR-HK/siRNA complexes achieved high anti-tumor activity through the downregulation of the Bcl-2 protein in mice, with an inhibition rate of 62.8%. Our data demonstrate that STR-HK is a highly promising siRNA delivery vector for therapeutic applications.

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Keywords
siRNA delivery; Cell penetrating peptide; Stearic acid; Nuclear localization sequence; Intratumoral injection
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A novel peptide for efficient siRNA delivery in vitro and therapeutics in vivo
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 21, 15 July 2015, Pages 74–84
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us