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Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect

Paper ID Volume ID Publish Year Pages File Format Full-Text
2933 143 2015 7 PDF Available
Title
Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect
Abstract

•Evaluation of antifungal effect of T. vulgaris and C. cassia against A. flavus.•Application of logistic kinetic model to the experimental data for optimal ratio calculation.•Optimal mixture has the synergistic effect with lower MIC and MFC values.•Modeling of results can advance synergistic studies by predicting optimal mixture.

The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT. vulgaris) and Cinnamomum cassia L. (EOC. cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT. vulgaris and EOC. cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated.Kinetic model revealed optimal ratio EOT. vulgaris: EOC. cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90 min), followed by 7:1 (110 min) and 1:1 (180 min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations.

Keywords
ATCC, American Type Culture Collection; EOC.cassia, Cinnamommum cassia essential oil; EOs, essential oils; EOT.vulgaris, Thymus vulgaris essential oil; DMSO, dimethyl sulfoxide; FBS, fetal bovine serum; FICI, fraction inhibitory concentration index; IA, i
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Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 99, 15 July 2015, Pages 131–137
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us