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Ionic strength effects on the photodegradation reactions of riboflavin in aqueous solution

Paper ID Volume ID Publish Year Pages File Format Full-Text
29376 44372 2016 7 PDF Available
Title
Ionic strength effects on the photodegradation reactions of riboflavin in aqueous solution
Abstract

•A unique effect of ionic strength on riboflavin (RF) photodegradation is shown.•Change in kinetic behavior of RF in phosphate buffer at same ionic strength occurs.•Effect of ionic strength on RF photoreduction is greater than that of photoaddition.•NaCl may form a nonfluorescent complex with RF to promote photodegradation reaction.•A charged species is involved in rate determining step of photodegradation reactions.

A study of the effect of ionic strength on the photodegradation reactions (photoreduction and photoaddition) of riboflavin (RF) in phosphate buffer (pH 7.0) has been carried out using a specific multicomponent spectrometric method. It has been found that the rates of photodegradation reactions of RF are dependent upon the ionic strength of the solutions at different buffer concentrations. The apparent first-order rate constants (kobs) for the photodegradation of riboflavin at ionic strengths of 0.1–0.5 (0.5 M phosphate) lie in the range of 7.35–30.32 × 10− 3 min− 1. Under these conditions, the rate constants for the formation of the major products, lumichrome (LC) by photoreduction pathway, and cyclodehydroriboflavin (CDRF) by photoaddition pathway, are in the range of 3.80–16.03 and 1.70–6.07 × 10− 3 min− 1, respectively. A linear relationship has been observed between log kobs and √ μ/1 + √ μ. A similar plot of log k/ko against √ μ yields a straight line with a value of ~+1 for ZAZB showing the involvement of a charged species in the rate determining step. NaCl appears to promote the photodegradation reactions of RF probably by an excited state interaction. The implications of ionic strength on RF photodegradation by different pathways and flavin–protein interactions have been discussed.

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Keywords
Riboflavin; Photodegradation mode; Photoproducts; Spectrometric assay; Ionic strength effect
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Ionic strength effects on the photodegradation reactions of riboflavin in aqueous solution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 157, April 2016, Pages 113–119
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us