fulltext.study @t Gmail

Aging effect and antibody immobilization on COOH exposed surfaces designed for dengue virus detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
2939 143 2015 10 PDF Available
Title
Aging effect and antibody immobilization on COOH exposed surfaces designed for dengue virus detection
Abstract

•Polymethacrylate coatings with surface carboxyl groups.•Stable surface chemistry over extended periods of time.•Surface immobilization of dengue antibody molecules and subsequent virus detection.

Polymethylmethacrylate-co-methacrylic acid, poly(MMA-co-MAA) coatings were produced with different initial molar ratios of monomers (MMA and MAA) in free-radical polymerization reaction. Polymeric platforms were specifically designed with controlled concentration of surface-exposed carboxyl (COOH) groups that can be used as a desirable functionality for protein immobilization. Spin-coated chips were used for antibody (Ab) immobilization in order to investigate the influence of COOH surface concentration on dengue virus detection efficiency in enzyme-linked immunosorbent assay (ELISA) experiment. Successful immobilization of Ab was achieved by two different techniques: (1) physical adsorption; and (2) covalent immobilization by carbodiimide coupling between the surface COOH groups and amine functionalities of dengue Ab molecules. Produced polymer coatings were characterized with surface spectroscopy techniques (Raman and X-ray photoelectron spectroscopy, XPS) and water-in-air contact angle (WCA) measurements. In particular, this research concentrated on the aging effect on the availability and activity of surface COOH groups. For that reason, WCA and Ab immobilization (ELISA) experiments were repeated on coated biochips after 3, 6 and 9 months of storage. Results in this paper describe the robust and sustainable functionalized polymeric platform that can be used effectively for protein activation and development of novel biosensors.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Polymer coatings; Surface functional groups; Immobilization; Enzyme activity; Immunoassay; Biosensors
First Page Preview
Aging effect and antibody immobilization on COOH exposed surfaces designed for dengue virus detection
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 99, 15 July 2015, Pages 183–192
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us