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DNA induced sequestration of a bioactive cationic fluorophore from the lipid environment: A spectroscopic investigation

Paper ID Volume ID Publish Year Pages File Format Full-Text
29392 44380 2016 8 PDF Available
Title
DNA induced sequestration of a bioactive cationic fluorophore from the lipid environment: A spectroscopic investigation
Abstract

•Binding interactions of PSF with lipid and ctDNA have been investigated independently.•Effect of DNA on the lipid bound probe is explored.•In the presence of DNA, the probe is released from the lipid environment and intercalated within DNA.•Competitive binding is ascribed to be responsible for the sequestration.

The effect of calf-thymus DNA (ctDNA) on the lipid bound probe, formed by the cationic phenazinium dye phenosafranin (PSF) and the anionic lipid dimyristoyl-L-α-phosphatidylglycerol (DMPG), has been unearthed exploiting various spectroscopic techniques. Steady state and time-resolved fluorometric studies and measurements of circular dichroism and DNA helix melting temperature reveal that in the presence of DNA the probe is dislodged from the lipid environment and gets intercalated within the DNA helix. The work qualitatively illustrates that the anionic lipid can be used as a potential nanocarrier for delivering the cationic drugs to the most relevant biomacromolecular target, DNA.

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Keywords
Phenosafanin; Lipid; ctDNA; Fluorescence; Circular dichroism; Helix melting
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DNA induced sequestration of a bioactive cationic fluorophore from the lipid environment: A spectroscopic investigation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 154, January 2016, Pages 118–125
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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