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Photoprotective effect of coumarin and 3-hydroxycoumarin in sea urchin gametes and embryonic cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
29399 44384 2015 8 PDF Available
Title
Photoprotective effect of coumarin and 3-hydroxycoumarin in sea urchin gametes and embryonic cells
Abstract

•Coumarin inhibited sea urchin embryonic development in a concentration-dependent manner from 5 μM.•Coumarin derivatives inhibited embryonic development 10 times more efficiently than coumarin.•Coumarin and 3-hydroxycoumarin protect sea urchin eggs and zygotes from UVB exposure.•Photoprotective effect of coumarin and 3-hydroxycoumarin was observed only when treatment was performed before irradiation.

Ultraviolet radiation B (UVB) represents 5% of all solar UV radiation and chronic exposure can induce harmful biological responses, including skin cancer. Prospection of new drugs with photoprotective properties and less toxic effects is constant and natural products have been the main options in this field. Coumarins are a group of natural phenolic compounds that shows several pharmacological activities. The aim of present work was to investigate the effect of coumarin and six derivatives in sea urchin gametes and zygotes exposed to UVB. Embryonic development assay was used to monitor UVB embryotoxicity. Firstly, we demonstrated that coumarin inhibited first embryonic cell division from 5 μM (EC50 = 52.9 μM) and its derivatives showed an embryotoxic effect ten times higher. Then, gametes or zygotes were treated with coumarin compounds before or after UVB exposure (UVB doses ranged from 0.056 to 0.9 kJ m−2). Pretreatment of gametes or zygotes with coumarin or 3-hydroxycoumarin (1 μM, both) decreased UVB embryotoxic effect. Protective effect of the compounds was observed only when cells were treated previous to UVB exposure. Coumarin derivatives 4-hydroxycoumarin, 6-hydroxycoumarin, 7-hydroxycoumarin, 6,7-dihydroxycoumarin and 6-methoxy-7-hydroxycoumarin did not exhibit photoprotective activity. Our data provides evidences that coumarin and 3-hydroxycoumarin can be a promising class of photoprotective drugs.

Graphical abstractPhotoprotective effect of coumarin and 3-hydroxycoumarin on sea urchin spermatozoa, eggs or zygotes exposed to UVB.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
UVB; Coumarin; 3-Hydroxycoumarin; Photoprotective; Sea urchin
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Photoprotective effect of coumarin and 3-hydroxycoumarin in sea urchin gametes and embryonic cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 146, May 2015, Pages 44–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us