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Phloem mobility and translocation of fluorescent conjugate containing glucose and NBD in castor bean (Ricinus communis)

Paper ID Volume ID Publish Year Pages File Format Full-Text
29415 44395 2014 7 PDF Available
Title
Phloem mobility and translocation of fluorescent conjugate containing glucose and NBD in castor bean (Ricinus communis)
Abstract

•Fluorescent glucose-insecticide conjugate.•Trace the phloem mobility and translocation of fluorescent conjugates in castor bean seedling.•Fluorescent labeling techniques could be a valuable tool to investigate pesticide movement in plants.

Phloem mobility is an important factor for long-distance transport of systemic pesticides in plants. Our previous study revealed that a fluorescent glucose-insecticide conjugate, N-{3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-iodo-1H-pyrazol-5-yl}-N-{[1-(β-D-glucopyranosyl)-1H-1,2,3-triazole-4-yl]methyl}-N-{[1-((N-(7-nitrobenz-2-oxa-1,3-diazole-4-amine))-propyl)-1H-1,2,3-triazole-4-yl]methyl}amine (IPGN), can be transported in tobacco cells. Several studies have also indicated that glucose moieties can guide the conjugates into plant cells. In this study, we investigated the phloem mobility of IPGN within castor bean seedlings. Cotyledon uptake experiment results show that IPGN could enter the phloem of the mid-veins of cotyledons. The results of further quantitative analysis show that IPGN was present in small amounts in the phloem sap despite the inconsistencies of physicochemical properties with diffusion through the plasma membrane. Its concentration in the phloem sap (about 370 nM at 5 h) was much lower than that in the incubation medium (100 μM), which suggests that IPGN exhibited weak phloem mobility. After the leaves of Ricinus plantlets were treated with IPGN, green fluorescence could be observed in the phloem of the petioles, bud apical nodes, bud mid-veins, and mid-veins of the untreated leaves. The localization of the fluorescent conjugate at various levels of Ricinus plantlets indicates that it was translocated at a distance to sink organs via sieve tubes. The results proved that introducing a glucose group is a feasible approach to modify non-phloem-mobile pesticides and produce phloem-mobile pesticides.

Keywords
Fluorescence microscopy; Fluorescent glucose-conjugate; Phloem mobility; Translocation; Visualization
First Page Preview
Phloem mobility and translocation of fluorescent conjugate containing glucose and NBD in castor bean (Ricinus communis)
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 132, 5 March 2014, Pages 10–16
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering