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Synthesis, crystal structures, in vitro DNA binding, antibacterial and cytotoxic activities of new di- and polynuclear silver(I) saccharinate complexes with tertiary monophosphanes

Paper ID Volume ID Publish Year Pages File Format Full-Text
29431 44397 2014 12 PDF Available
Title
Synthesis, crystal structures, in vitro DNA binding, antibacterial and cytotoxic activities of new di- and polynuclear silver(I) saccharinate complexes with tertiary monophosphanes
Abstract

•New silver(I) saccharinate complexes with tertiary monophosphanes were synthesized and structurally characterized.•Complexes bind to DNA via intercalation.•Complexes exhibit high cytotoxic activity against various cancer cells.•Complexes show remarkable antibacterial activity against various bacteria.

Four new silver(I) saccharinate (sac) complexes, [Ag(μ-sac)(PPh3)]2 (1), [Ag(μ-sac)(PPh2Cy)]2 (2), [Ag(μ-sac)(PPhCy2)]2 (3) and [Ag(μ-sac)(PCy3)]n (4), where PPh3 = triphenylphosphane, PPh2Cy = cyclohexyldiphenylphosphane, PPhCy2 = dicyclohexylphenylphosphane and PCy3 = tricyclohexylphosphane, have been synthesized and fully characterized by elemental analysis, IR, NMR, ESI-MS and single crystal X-ray diffraction. Fluorescence ethidium bromide displacement indicate that all complexes bind to fish sperm (FS) DNA by intercalation with binding constants (KA) of 29.1 ± 0.26 × 105 M−1 for 1, 2.54 ± 0.12 × 105 M−1 for 2, 2.42 ± 0.08 × 105 M−1 for 3, 0.19 ± 0.03 × 105 M−1 for 4. The relative viscosities of the FS-DNA solutions increase with increasing of the complex concentration, providing strong evidence for the intercalation mode. The gel electrophoresis assay further confirms their binding with the pBR322 plasmid DNA. The MIC values of the silver(I) complexes are generally higher than those of AgNO3 and silver sulfadiazine, but 1 presents a promising activity against Salmonellatyphimurium and Staphylococcusaureus. All complexes are highly cytotoxic on human lung carcinoma (A549) and human breast adenocarcinoma (MCF-7) cell lines with IC50 values ranging from 0.82 to 3.13 μM.

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Keywords
Silver(I); Saccharinate; Phosphine; DNA binding; Antibacterial activity; Cytotoxic activity
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Synthesis, crystal structures, in vitro DNA binding, antibacterial and cytotoxic activities of new di- and polynuclear silver(I) saccharinate complexes with tertiary monophosphanes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 131, 5 February 2014, Pages 31–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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