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Protective role of extracellular catalase (KatA) against UVA radiation in Pseudomonas aeruginosa biofilms

Paper ID Volume ID Publish Year Pages File Format Full-Text
29433 44397 2014 12 PDF Available
Title
Protective role of extracellular catalase (KatA) against UVA radiation in Pseudomonas aeruginosa biofilms
Abstract

•KatA activity is present in the matrix of P. aeruginosa biofilms.•Extracellular KatA is essential in the defense of P. aeruginosa biofilms against UVA.•KatA is not involved in the resistance to photocatalysis of P. aeruginosa biofilms.

One of the more stressful factors that Pseudomonas aeruginosa must face in nature is solar UVA radiation. In this study, the protective role of KatA catalase in both planktonic cells and biofilms of P. aeruginosa against UVA radiation was determined by using the wild-type (PAO1) and an isogenic catalase deficient strain (katA). The katA strain was more sensitive than the wild-type, especially in the case of biofilms. Moreover, the wild-type biofilm was more resistant than its planktonic counterpart, but this was not observed in the katA strain. Striking KatA activity was detected in the matrix of katA+ strains, and to our knowledge, this is the first report of this activity in the matrix of P. aeruginosa biofilms. Provision of bovine catalase or KatA to the matrix of a katA biofilm significantly increased its UVA tolerance, demonstrating that extracellular KatA is essential to optimal defense against UVA in P. aeruginosa biofilms. Efficiency of photocatalytic treatments using TiO2 and UVA was lower in biofilms than in planktonic cells, but KatA and KatB catalases seem not to be responsible for the higher resistance of the sessile cells to this treatment.

Keywords
Pseudomonas; Biofilms; Ultraviolet-A; UVA; Catalase; KatA
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Protective role of extracellular catalase (KatA) against UVA radiation in Pseudomonas aeruginosa biofilms
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 131, 5 February 2014, Pages 53–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us