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In vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract

Paper ID Volume ID Publish Year Pages File Format Full-Text
29434 44397 2014 9 PDF Available
Title
In vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract
Abstract

•Standardization of SPF commercial sunscreens versus cell viability.•Assess the photoprotection of G. brasiliensis extract by standardized method.•Correlation of in vitro results with in vivo methods.

The damaging effects of sunlight to the skin has triggered studies that involve the synthesis and extraction of organic compounds from natural sources that can absorb UV radiation, and studies on polyphenolic compounds with antioxidant and anti-inflammatory properties that can be used as photochemopreventive agents for reducing skin damage. We investigated the in vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract (GbEE). We evaluated the cell viability of L929 fibroblasts after UVB exposure using a quartz plate containing the extract solution or the GbEE formulation. The in vivo photoprotective effect of the GbEE formulation was evaluated by measuring the UVB damage-induced decrease in endogenous reduced glutathione (GSH), the increase in myeloperoxidase (MPO) activity and secretion of cytokines IL-1β and TNF-α. The in vitro methodology using fibroblasts showed that the photoprotective properties of the GbEE solutions and 10% GbEE formulation were similar to the commercial sunscreen (SPF-15). In vivo results demonstrated of the GbEE formulation in decreasing UVB induced-damage such as GSH depletion, an increased in MPO activity and secretion of cytokines IL-1β and TNF-α. The results showed that the extract has great potential for use as a sunscreen in topical formulations in addition to UV filters.

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Keywords
Garcinia brasiliensis; Antioxidant; Anti-inflammatory; Cytotoxicity; Photoprotection; Photochemoprotection; In vitro SPF
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In vitro and in vivo photoprotective/photochemopreventive potential of Garcinia brasiliensis epicarp extract
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 131, 5 February 2014, Pages 65–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us