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Study on the interaction of sulforaphane with human and bovine serum albumins

Paper ID Volume ID Publish Year Pages File Format Full-Text
29450 44405 2013 7 PDF Available
Title
Study on the interaction of sulforaphane with human and bovine serum albumins
Abstract

Sulforaphane; [1-isothiocyanato-4-(methylsulfinyl) butane], (SFN) is an isothiocyanate derived from glucoraphanin present in cruciferous vegetables and has a variety of potential chemopreventive actions. This study was designed to examine the interaction of sulforaphane with HSA and BSA. FTIR, UV–Vis spectroscopic methods as well as molecular modeling were used to determine the drug binding mode, binding constant and the effect of drug complexation on serum albumins stability and conformation. Structural analysis showed that SFN bind HSA and BSA via polypeptide polar groups with overall binding constants of KSFN–HSA = 6.54 × 104 and KSFN–BSA = 8.55 × 104 M−1. HSA and BSA conformations were altered by a major reduction of α-helix upon SFN interaction. These results suggest that serum albumins might act as carrier proteins for SFN in delivering them to target tissues.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► SFN binds HSA via non-polar amino acids. ► SFN binds BSA via polar amino acids. ► SFN interacts with the positively charged amino acids. ► The stronger binding was observed for BSA than HSA. ► HSA and BSA might act as carrier proteins for SFN in delivering it to target molecules.

Keywords
HSA, human serum albumin; BSA, bovine serum albumin; SFN, sulforaphane; FTIR, Fourier transform infrared; UV–Vis, ultraviolet–visibleHSA; BSA; Sulforaphane; FTIR; UV–Visible spectroscopy; Docking
First Page Preview
Study on the interaction of sulforaphane with human and bovine serum albumins
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 122, 5 May 2013, Pages 61–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering