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Near-infrared fluorescing IR820-chitosan conjugate for multifunctional cancer theranostic applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
29459 44409 2013 8 PDF Available
Title
Near-infrared fluorescing IR820-chitosan conjugate for multifunctional cancer theranostic applications
Abstract

This study reports the preparation and characterization of IR820-chitosan conjugates that have potential multifunctional imaging-hyperthermia applications in cancer. The conjugates were formulated by covalent coupling of chitosan to a carboxyl derivatized IR820, and studied for optical imaging and hyperthermia applications. IR820-chitosan conjugates were able to generate heat upon exposure to 808 nm laser and produce hyperthermic cell growth inhibition in cancer cell lines MES-SA, SKOV-3 and Dx5. The level of cell growth inhibition caused by hyperthermia was significantly higher for IR820-chitosan compared to IR820 in MES-SA and Dx5 cells. Fluorescent microscope images of these cancer cell lines after 3-h exposure to 5 μM IR820-chitosan showed that the conjugates can be used for in vitro near-infrared imaging. In an in vivo rat model, the conjugates accumulated in the liver after i.v. injection and were excreted through the gastrointestinal tract, demonstrating a different biodistribution when compared to the free dye. The accumulation of these conjugates in bile with subsequent gastrointestinal excretion allows for potential applications as gastrointestinal contrast agents and delivery vehicles. This formulation can potentially be used in multifunctional cancer theranostics.

► We successfully conjugated IR820 (a near-infrared dye) to chitosan polymer. ► The conjugate exhibited potential to be used as in vitro and in vivo imaging agent. ► When exposed to laser, the conjugate caused cancer cell growth inhibition in vitro. ► IR820-chitosan exhibited strong accumulation in the intestines after 24 h. ► The conjugate shows potential for multifunctional gastrointestinal applications.

Keywords
Cancer; Chitosan; Hyperthermia; Imaging; IR820; Theranostics
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Near-infrared fluorescing IR820-chitosan conjugate for multifunctional cancer theranostic applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 119, 5 February 2013, Pages 52–59
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us