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Effects of perfluorooctane sulfonate on the conformation and activity of bovine serum albumin

Paper ID Volume ID Publish Year Pages File Format Full-Text
29472 44411 2016 8 PDF Available
Title
Effects of perfluorooctane sulfonate on the conformation and activity of bovine serum albumin
Abstract

•Binding interaction of PFOS with BSA was investigated.•PFOS exhibits influence on the conformation of BSA.•PFOS increases the protein stability of BSA during the thermal denaturation of protein.•Multiple non-covalent binding forces are involved in the binding process.

Perfluorooctane sulfonate (PFOS) is among the most prominent contaminates in human serum and has been reported to possess potential toxicity to the human body. In this study, the effects of PFOS on the conformation and activity of bovine serum albumin (BSA) were investigated in vitro. The results indicated that the binding interaction of PFOS with BSA destroyed the tertiary and secondary structures of protein with the loss of α-helix structure and the increasing of hydrophobic microenvironment of the Trp or Tyr residues. During the thermal denaturation protein, PFOS increases the protein stability of BSA. The proportion of α-helix decreased on increasing the PFOS concentration and the microenvironment of the Trp or Tyr residues becomes more hydrophobic. The results from molecular modeling indicated that BSA had not only one possible binding site to bind with PFOS by the polar interaction, hydrogen bonds and hydrophobic forces. In addition, the BSA relative activities were decreased with the increase of PFOS concentration. Such loss of BSA activity in the presence of PFOS indicated that one of the binding sites in BSA is located in subdomain IIIA, which is in good agreement with the fluorescence spectroscopic experiments and molecular modeling results. This study offers a comprehensive picture of the interactions of PFOS with serum albumin and provides insights into the toxicological effect of perfluoroalkylated substances.

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Keywords
Perfluorooctane sulfonate; Serum albumin; Secondary structure; Denaturation; Toxicological evaluation
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Effects of perfluorooctane sulfonate on the conformation and activity of bovine serum albumin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 159, June 2016, Pages 66–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us