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Recombinant protein production in Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter: From carbon source metabolism to bioreactor operation parameters

Paper ID Volume ID Publish Year Pages File Format Full-Text
2948 144 2015 17 PDF Available
Title
Recombinant protein production in Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter: From carbon source metabolism to bioreactor operation parameters
Abstract

•We review the current state of art on r-protein production by Pichia pastoris under PGAP.•Bioprocess parameters and strategies are analysed for r-protein production.•Carbon source and oxygen modulate r-protein production under PGAP.•Requirement for the studies on molecular basis of PGAP regulation is discussed.•New strategies for the enhanced production of r-proteins under PGAP are discussed.

The yeast Pichia (Komagataella) pastoris has become a potential host system for recombinant protein (r-protein) production. Several strong inducible and constitutive promoters have been identified in P. pastoris which make the host ideal in r-protein expressions. The constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene, PGAP, is promising as its use offers the advantage of associating product quantity to growth which can be optimized by a well-designed feeding strategy so long as the product itself is not toxic to the host. In this article, carbon source metabolism and the central carbon pathways of P. pastoris are reviewed. After comparing PGAP with the available promoters of P. pastoris, the bioreactor operation parameters and bioreactor operation strategies are analyzed for r-protein production under PGAP by focusing on response of the cells, final product concentration, yield, and productivity. The overview of the subject reveals the requirement for in-depth information on the overall regulation of P. pastoris metabolism that can enable fine-tuning bioreactor operation parameters in relation with the physiology of the microorganism in order to develop new strategies for the enhanced r-protein production.

Keywords
Bioreactors; Fed-batch culture; Bioprocess design; Fermentation; Pichia pastoris; GAP promoter
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Recombinant protein production in Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter: From carbon source metabolism to bioreactor operation parameters
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 95, 15 March 2015, Pages 20–36
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us