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Spectroscopic investigations on partial intercalative binding behaviour of terpyridine based copper(II) complexes with DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
29483 44411 2016 9 PDF Available
Title
Spectroscopic investigations on partial intercalative binding behaviour of terpyridine based copper(II) complexes with DNA
Abstract

•Novel tertiary pyridine ligand (meFtpy) was synthesized and characterized.•Single crystals of corresponding mixed ligand copper (II) complexes were obtained.•DNA binding ability of the complexes was compared with doxorubicin.•Partial intercalative binding mode of the complexes with DNA was assessed.

Copper based metal complexes have been studied extensively towards DNA interaction aspects, the possible interactions being at the major or minor grooves, intercalation between base pairs, etc. The nature of the ligand decides the binding mode of the complexes thereby exerting different biological significance. Based on this, we have synthesized two mixed ligand copper(II) complexes, [Cu(meFtpy)(bpy)](NO3)2.2(H2O) (1) and [Cu(meFtpy)(phen)](NO3)2.(H2O) (2) based on new furfuryl substituted tertiary pyridine ligand (meFtpy) and ancillary ligands (phen, bpy). They are characterized by UV Visible, FT-IR, 1H & 13C NMR and mass spectroscopic techniques. The structures of both the complexes are confirmed by single crystal XRD revealing triclinic crystal system showing penta coordination possessing distorted square pyramidal geometry. The binding ability of the complexes has been explored based on the results of DNA binding studies assessed by different spectroscopic techniques like UV absorption titration, fluorescence displacement assay and circular dicroism and found to show partial intercalative behaviour. The binding constant (Kb) values as obtained from UV absorption titration are found to be 1.29 × 104 M− 1 for 1 and 1.46 × 104 M− 1 for 2 and are compared with the values obtained for doxorubicin, a partial intercalator drug. The binding affinities obtained from absorption titration are found in the order as Kb(doxo) > Kb(2) > Kb(1) > Kb(meFtpy).

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Keywords
Tertiary pyridine; DNA binding; Partial intercalation; Docking; Copper complexes
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Spectroscopic investigations on partial intercalative binding behaviour of terpyridine based copper(II) complexes with DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 159, June 2016, Pages 133–141
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us