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In vitro evaluation of combined hyperthermia and photodynamic effects using magnetoliposomes loaded with cucurbit [7]uril zinc phthalocyanine complex on melanoma

Paper ID Volume ID Publish Year Pages File Format Full-Text
29499 44415 2012 4 PDF Available
Title
In vitro evaluation of combined hyperthermia and photodynamic effects using magnetoliposomes loaded with cucurbit [7]uril zinc phthalocyanine complex on melanoma
Abstract

The aims of this study were two fold; to develop magnetoliposomes (MLs) loaded with zinc phthalocyanine (ZnPc) complexed with cucurbituril (CB) (CB:ZnPc-MLs) and to evaluate their in vitro photodynamic (PD) and/or hyperthermia (HT) effects while using melanoma cells (B16-F10) as model. The liposomal formulations were characterized by both average diameter and zeta potential. The vesicle average size ranged from 150 to 200 nm and the polydispersity index (PdI) from 0.093 to 0.230. The zeta potential was significantly positive with values between 48 and 57 mV. The cell viability (CV) after PD and HT treatments was assessed by colorimetric MTT method. Melanoma cells were initially treated with the liposome formulation without light and magnetic field application, revealing cell viability not different from the control cells (p > 0.05). Photodynamic and hyperthermia assays were also applied separately, demonstrating that PD is more effective than HT in reducing the CV of the neoplastic cells. Combined application of both PD and HT treatments was even more effective in reducing the CV of B16-F10 cells. At the highest light dose (2 J/cm2) and under magnetic field activation the CV was about half than PD applied alone. Therefore, the use of the photosensitizer-loaded magnetoliposome for combined photodynamic therapy (PDT) and magnetohyperthermia (MHT) application can be considered as a potential tool to treat malignant melanoma.

► Magnetohyperthermia has been used in combination with photodynamic therapy. ► We evaluated in vitro synergic effect of both therapies on melanoma cells. ► The combination of such therapies led to decrease in cell viability of melanoma.

Keywords
Hyperthermia; Photodynamic therapy; Cationic liposome; Magnetic fluid; Zinc phthalocyanine; Melanoma
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In vitro evaluation of combined hyperthermia and photodynamic effects using magnetoliposomes loaded with cucurbit [7]uril zinc phthalocyanine complex on melanoma
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 115, 3 October 2012, Pages 1–4
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us