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Contribution of UVB radiation to bacterial inactivation by natural sunlight

Paper ID Volume ID Publish Year Pages File Format Full-Text
29507 44415 2012 5 PDF Available
Title
Contribution of UVB radiation to bacterial inactivation by natural sunlight
Abstract

The contribution of different components of sunlight to the lethal action exerted by this radiation on bacteria was studied using Pseudomonas aeruginosa ATCC27853 as a model organism. When solar UVB was excluded from the incident radiation by filtering it through a naphthalene solution (cut off 327 nm), significant modifications were observed in the cell-death kinetics. These modifications were comparable to those expected for a reduction of 27–32% in the dose rate, according to the model used in the analysis of the survival curves, and were also observed when the effects of sunlight filtered through polyethylene terephthalate (cut off 331 nm) or polystyrene (cut off 298 nm) were compared. Viability of P. aeruginosa remained almost unchanged when the incident radiation was filtered through a sodium nitrite solution (cut off 406 nm) in order to exclude the UVA and UVB components of sunlight. Nevertheless, a delay in colony formation was detected in bacteria treated in this way, suggesting that a non-lethal effect was exerted by visible light. The results are not consistent with a generally accepted notion which attributes the lethal action of sunlight to the radiation with wavelengths above 320 nm. The characterization of UVB contribution to the lethal effect of sunlight on bacteria is relevant for understanding of the mechanism of cell death, and for improvement of dosimetry techniques and irradiation procedures.

► The contribution of UVB to bacterial inactivation by sunlight was evaluated. ► The effect of solar UVB on bacterial survival is significant. ► Filtration through polyethylene terephthalate eliminates the effect of solar UVB. ► Effects of UVB should be considered in the design of sunlight irradiations.

Keywords
Sunlight; Ultraviolet; Survival; Pseudomonas
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 115, 3 October 2012, Pages 58–62
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us