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Reversal of iC3b-inhibited dendritic cell differentiation via inhibition of the extracellular signal-regulated mitogen-activated protein kinase promotes CD4+ T cell proliferation

Paper ID Volume ID Publish Year Pages File Format Full-Text
29534 44419 2012 9 PDF Available
Title
Reversal of iC3b-inhibited dendritic cell differentiation via inhibition of the extracellular signal-regulated mitogen-activated protein kinase promotes CD4+ T cell proliferation
Abstract

ObjectivesTo investigate the roles of ERK1/2 and p38 MAPK cascades in the differentiation of iC3b-combined CD14+ monocyte into CD1a+ MDDC, and to study how these cells influence CD4+ T cell proliferation.MethodsCD14+ monocyte was co-cultured with iC3b with or without inhibitors specific for ERK1/2 or p38 MAPK pathways for 2 days, then the expressions of CD14, CD1a, phophso-ERK1/2, phophso-p38, IL-10 and IL-12 p70 were detected, and CD4+ T cell proliferation was measured via 3H-TdR as well.ResultsMaturation of CD1a+ DC was inhibited by iC3b along with downregulated expressions of CD1a, phophso-p38 and IL-12p70 and upregulated expressions of phophso-ERK1/2 and IL-10, and the CD4+ T cell proliferation was restrained accordingly. When pretreated with inhibitor specific for ERK1/2 pathway, the inhibited maturation of imDC was reversed prominently with a higher level expression of CD1a and IL-12p70, whereas expressions of phophso-ERK1/2 and IL-10 were lowered, and accordingly the CD4+ T cell proliferation restored significantly.ConclusionsiC3b inhibited the differentiation of CD14+ monocytes into CD1a+ MDDCs via ERK1/2 pathway, and restoration of CD1a+ MDDCs maturation occurred with the treatment of inhibitors specific for ERK1/2 pathway. Meanwhile, treatment of the inhibitor for the ERK1/2 cascade reversed the inhibited CD4+ T cell proliferation, implying a potential possibility for clinical intervention.

► iC3b inhibited maturation of CD1a+ MDDCs from CD14+ monocytes via ERK1/2 pathway. ► CD4+ T cell proliferation was interrupted with iC3b exposure. ► Treatment of ERK1/2 cascade inhibitor restored CD1a+ MDDCs maturation. ► The inhibitor treatment reversed the inhibited CD4+ T cell proliferation.

Keywords
iC3b; Dentritic cell; ERK1/2; CD4+ T cell; CD14+ monocyte
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Reversal of iC3b-inhibited dendritic cell differentiation via inhibition of the extracellular signal-regulated mitogen-activated protein kinase promotes CD4+ T cell proliferation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 111, 4 June 2012, Pages 50–58
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us