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Study of DNA–emodin interaction by FTIR and UV–vis spectroscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
29535 44419 2012 5 PDF Available
Title
Study of DNA–emodin interaction by FTIR and UV–vis spectroscopy
Abstract

Emodin, a plant- and fungus-derived anthraquinone, exerts genotoxic and antioxidative effects and shows promise in antitumor and antibacterial therapies. The aim of this study was to examine the molecular interactions of emodin with DNA in aqueous solution at physiological pH using spectroscopic methods. Fourier Transform Infrared (FTIR) Spectroscopy and UV absorption spectra were used to determine the structural features, the binding mode and the association constants. Our UV-spectroscopic results indicate that emodin interacts with DNA by intercalation and by external binding. FTIR results suggest that emodin interaction occurs preferably via adenine and thymine base pairs and also weakly with the phosphate backbone of the DNA double helix. The binding constant for emodin–DNA complex formation is estimated to be K = 5.59 × 103 M−1. No significant changes of DNA conformation were observed upon emodin–DNA complexation.

Graphical abstractFTIR and UV spectroscopic studies of emodin interaction with double helical structure of DNA.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Examine the molecular interactions of emodin with DNA in aqueous solution at physiological pH using spectroscopic methods. ► Emodin interacts with DNA by intercalation and by external binding. ► Emodin–DNA interaction occurs preferably via adenine and thymine base pairs. ► No significant changes of DNA conformation were observed upon emodin–DNA complexation. ► UV-spectroscopic results indicate intercalation of emodin into DNA.

Keywords
Anthraquinone; DNA; Intercalation; Binding constant; Binding mode
First Page Preview
Study of DNA–emodin interaction by FTIR and UV–vis spectroscopy
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 111, 4 June 2012, Pages 59–63
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering