fulltext.study @t Gmail

Antimicrobial photodynamic effect of extracts and oxoaporphine alkaloid isomoschatoline from Guatteria blepharophylla

Paper ID Volume ID Publish Year Pages File Format Full-Text
29576 44422 2016 9 PDF Available
Title
Antimicrobial photodynamic effect of extracts and oxoaporphine alkaloid isomoschatoline from Guatteria blepharophylla
Abstract

•Crude extracts, alkaloid fraction and isomoschatoline showed singlet oxygen production.•Isomoschatoline photodynamic activity happens predominantly through type II mechanism.•PDT effect against E. coli was significantly enhanced in the presence of additives.•Greater energy doses and double sample's dosage also decreased microbial survival.

Photodynamic Therapy, a tumor therapy idealized at the beginning of the last century, emerges nowadays as a promising treatment alternative against infectious diseases. In this study we report a bioguided study of Guatteria blepharophylla phytoderivatives for antimicrobial PDT. Crude extracts and fraction from the species bark were obtained and further fractionated for substances isolation. All samples were evaluated in relation to their photophysical (absorbance and fluorescence) and photochemical properties (1,3-DPBF bleaching method). Then, bioassays were conducted using as biological models bacteria and yeast strains and a diode laser as a light source. Phytochemical analyses lead to the isolation of 5 isoquinoline alkaloids from oxoaporphine subclass, denominated GB1 to GB5. Photophysical and photochemical analysis showed that extracts, fraction and GB1 (isomoschatoline) presented absorption profile with bands at 600–700 nm and were positive for singlet oxygen production. Photobiological assays indicate that these samples presented photodynamic antimicrobial activity against both gram-positive and gram-negative bacterial and some Candida ssp. yeast strains at sub-inhibitory concentrations. The susceptibility of gram-negative bacteria was significantly enhanced when CaCl2 or MgCl2 were employed. Greater energy doses and double sample's dosage also decreased microbial survival. It is suggested that GB1 photodynamic activity happens through both types I and II photochemical mechanisms, but with a predominance of the latter. Phytoderivatives of G. blepharophylla promoted antimicrobial effect, however more detailed study concerning chemical composition of the crude extracts and fractions as also photophysical and photochemical characteristics of GB1 are necessary to ensure their potential as photosensitizers at antimicrobial photodynamic inactivation.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
1,3-DPBF, 1,3-diphenylisobenzofuran; CFM, confocal fluorescent microscopy; GBCH, bark hexane extract; GBCM, bark methanol extract; GBFA, alkaloid fraction; HMBC, Heteronuclear Multiple Bond Correlation; HMQC, Heteronuclear Multiple Quantum Coherence; TLC,
First Page Preview
Antimicrobial photodynamic effect of extracts and oxoaporphine alkaloid isomoschatoline from Guatteria blepharophylla
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 160, July 2016, Pages 154–162
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us