fulltext.study @t Gmail

Gold nanoparticles biosensor of Brucella spp. genomic DNA: Visual and spectrophotometric detections

Paper ID Volume ID Publish Year Pages File Format Full-Text
2959 145 2015 7 PDF Available
Title
Gold nanoparticles biosensor of Brucella spp. genomic DNA: Visual and spectrophotometric detections
Abstract

•A hybridization assay for detecting Brucella spp. genomic DNA is presented.•Detection was performed in clinical samples.•Detection method is highly simple and visually.

IntroductionThe causative agents of brucellosis from genus Brucella can infect a variety of animal species and human. However, conventional methods for the detection of Brucella are time-consuming and the handling of microorganism is hazardous for laboratory personnel. In this study, a nanobiosensor based on gold nanoparticles (AuNPs) and oligonucleotide probe was designed for the visual detection of Brucella spp.MethodsA specific oligonucleotide probe from IS711 gene region was functionalized with AuNPs (AuNP-probe). Then, AuNP-probe was exposed to target (complementary) and non-target (non-complementary) DNA for the hybridization. Also, hybridization conditions were analyzed in the presence of Brucella genomic DNA and extracted DNA from clinical samples.ResultsUpon acid addition, a red color for the samples containing complementary DNA was observed, whereas in the samples without complementary DNA, AuNP-probe turned blue-purple. The results were investigated visually and also by UV–vis spectroscopic measurements. This method detected up to 1.09 pg μL−1 of unamplified Brucella genomic DNA. Since Brucella is a facultative intracellular bacteria, clinical samples were amplified by PCR, and colorimetric detections were performed. The results showed high sensitivity and selectivity for the amplified clinical samples.ConclusionTo the best of our knowledge, this developed assay is highly specific, and this is the first assay that can be used with low cost, and as a method of rapid, direct and visual detection of Brucella spp.

Keywords
Gold nanoparticles; Brucella spp.; Biosensors; Immobilization; DNA; Aggregation
First Page Preview
Gold nanoparticles biosensor of Brucella spp. genomic DNA: Visual and spectrophotometric detections
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 97, 15 May 2015, Pages 1–7
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us