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Artificial pheomelanin nanoparticles and their photo-sensitization properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
29595 44422 2016 6 PDF Available
Title
Artificial pheomelanin nanoparticles and their photo-sensitization properties
Abstract

•Pheomelanin-type nanoparticles (PMNPs) were successfully synthesized.•The synthesized PMNPs had a spherical shape of approximately 100 nm size.•The synthesized PMNPs exhibited high dispersion stability in various culture media.•PMNPs showed significant phototoxicity to HeLa cells under UV light.

Pheomelanin-type nanoparticles (PMNPs) were synthesized through a simple oxidative polymerization of 3,4-dihydroxyphenylalanine (DOPA) in the presence of cysteine by KMnO4. The synthesized PMNPs had a diameter of approximately 100 nm, exhibited high dispersion stability in neutral water and various culture media and possessed similar morphology to naturally occurring pheomelanins. The efficiency of photoinduced generation of hydroxyl radicals from PMNPs was determined and related in vitro cell experiments that were carried out, with data being compared to those from eumelanin-type nanoparticles (EMNPs) and natural sepia melanin nanoparticles. Endocytosed PMNPs showed the highest phototoxicity (~ 50% viability) to UV-irradiated HeLa cells, confirming the direct relationship between phototoxic efficiency and the generation of hydroxyl radicals through the complex processes of the O2 sensitization.

Graphical AbstractWater dispersible pheomelanin-type nanoparticles (PMNPs) were synthesized through a simple oxidative polymerization of 3,4-dihydroxyphenylalanine (DOPA) in the presence of cysteine and exhibited phototoxic efficiency compared to eumelanin-type nanoparticles (EMNPs).Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Photosensitization property; Pheomelanin nanoparticles; Water-dispersible melanin models
First Page Preview
Artificial pheomelanin nanoparticles and their photo-sensitization properties
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 160, July 2016, Pages 330–335
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering