fulltext.study @t Gmail

Photoreduction of the ferredoxin/ferredoxin–NADP+-reductase complex by a linked ruthenium polypyridyl chromophore

Paper ID Volume ID Publish Year Pages File Format Full-Text
29597 44422 2016 8 PDF Available
Title
Photoreduction of the ferredoxin/ferredoxin–NADP+-reductase complex by a linked ruthenium polypyridyl chromophore
Abstract

•Ruthenium derivatives were attached to 5 cysteine-substituted mutants of ferredoxin.•An electron transfer rate up to 2 × 107 s− 1 was measured from Ru* to ferredoxin.•Ferredoxin (Fd)–FNR interactions are functional with Ru bound to C67 of Fd.•First-order intracomplex electron transfer between Fd and FNR has been measured.

Photosynthetic ferredoxin and its main partner ferredoxin–NADP+-reductase (FNR) are key proteins during the photoproduction of reductive power involved in photosynthetic growth. In this work, we used covalent attachment of ruthenium derivatives to different cysteine mutants of ferredoxin to trigger by laser-flash excitation both ferredoxin reduction and subsequent electron transfer from reduced ferredoxin to FNR. Rates and yields of reduction of the ferredoxin [2Fe–2S] cluster by reductively quenched Ru* could be measured for the first time for such a low redox potential protein whereas ferredoxin–FNR electron transfer was characterized in detail for one particular Ru–ferredoxin covalent adduct. For this adduct, the efficiency of FNR single reduction by reduced ferredoxin was close to 100% under both first-order and diffusion-limited second-order conditions. Interprotein intracomplex electron transfer was measured unambiguously for the first time with a fast rate of c. 6500 s− 1. Our measurements point out that Ru photosensitizing is a powerful approach to study the functional interactions of ferredoxin with its numerous partners besides FNR.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Electron transfer; Photochemistry; Protein–protein interactions; Photosynthesis; Kinetics
First Page Preview
Photoreduction of the ferredoxin/ferredoxin–NADP+-reductase complex by a linked ruthenium polypyridyl chromophore
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 160, July 2016, Pages 347–354
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us