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Structure of the catalytic, inorganic core of oxygen-evolving photosystem II at 1.9 Å resolution

Paper ID Volume ID Publish Year Pages File Format Full-Text
29607 44425 2011 10 PDF Available
Title
Structure of the catalytic, inorganic core of oxygen-evolving photosystem II at 1.9 Å resolution
Abstract

The catalytic center for photosynthetic water-splitting consists of 4 Mn atoms and 1 Ca atom and is located near the lumenal surface of photosystem II. So far the structure of the Mn4Ca-cluster has been studied by a variety of techniques including X-ray spectroscopy and diffraction, and various structural models have been proposed. However, its exact structure is still unknown due to the limited resolution of crystal structures of PSII achieved so far, as well as possible radiation damages that might have occurred. Very recently, we have succeeded in solving the structure of photosystem II at 1.9 Å, which yielded a detailed picture of the Mn4CaO5-cluster for the first time. In the high resolution structure, the Mn4CaO5-cluster is arranged in a distorted chair form, with a cubane-like structure formed by 3 Mn and 1 Ca, 4 oxygen atoms as the distorted base of the chair, and 1 Mn and 1 oxygen atom outside of the cubane as the back of the chair. In addition, four water molecules were associated with the cluster, among which, two are associated with the terminal Mn atom and two are associated with the Ca atom. Some of these water molecules may therefore serve as the substrates for water-splitting. The high resolution structure of the catalytic center provided a solid basis for elucidation of the mechanism of photosynthetic water splitting. We review here the structural features of the Mn4CaO5-cluster analyzed at 1.9 Å resolution, and compare them with the structures reported previously.

► Structure of the Mn4CaO5-cluster recently analyzed at 1.9 Å resolution was reviewed. ► The high resolution structure was compared with the previously reported ones. ► All of the ligands for the metal cluster were identified and discussed. ► Possible roles of three residues in the second coordination sphere were discussed. ► Additional Ca2+ and Cl- ions revealed in the high resolution structure were discussed.

Keywords
Crystal structure; Membrane protein structure; Oxygen-evolving complex; Photosystem II; Water-oxidation
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Structure of the catalytic, inorganic core of oxygen-evolving photosystem II at 1.9 Å resolution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 104, Issues 1–2, July–August 2011, Pages 9–18
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us