fulltext.study @t Gmail

Kinetics of enzymatic synthesis of monoferuloyl glycerol and diferuloyl glycerol by transesterification in [BMIM]PF6

Paper ID Volume ID Publish Year Pages File Format Full-Text
2962 145 2015 7 PDF Available
Title
Kinetics of enzymatic synthesis of monoferuloyl glycerol and diferuloyl glycerol by transesterification in [BMIM]PF6
Abstract

•Max yields of monoferuloyl glycerol (63.72 ± 1.26%) and diferuloyl glycerol (78.80 ± 2.09%) were achieved.•The activation energy for ethyl ferulate conversion was 40.16 kJ/mol.•The activation energies for monoferuloyl glycerol and diferuloyl glycerol formations were 31.43, and 85.38 kJ/mol, respectively.•Michaelis constants for ethyl ferulate and glycerol were 0.23 and 0.42 mol/L, respectively.•Transesterification mechanism with the inhibitions of ethyl ferulate and glycerol was proposed.

Feruloyl glycerols (FGs), water-soluble glycerides (monoferuloyl glycerol (MFG) and diferuloyl glycerol (DFG)) of ferulic acid, can be used as natural ultraviolet (UV) filters and antioxidants in chemical, food, pharmaceuticals, and drug industries. In order to promote the synthesis of FGs, the effect of process parameters, optimization, thermodynamic and kinetic properties on the enzymatic transesterification of ethyl ferulate (EF) with glycerol in [BMIM]PF6 were investigated. The maximum yields of MFG (63.72 ± 1.26%) and DFG (78.80 ± 2.09%) were achieved at low glycerol concentrations in [BMIM]PF6. The activation energies for EF conversion and transesterification to form MFG and DFG were calculated as 40.16, 31.43 and 85.38 kJ/mol, respectively, based on the Arrhenius law. Reaction kinetics agreed with the Ping–Pong Bi–Bi mechanism with the inhibitions of EF and glycerol. The enzymatic mechanism of the transesterification of EF with glycerol in [BMIM]PF6 was also proposed.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
FA, ferulic acid; EF, ethyl ferulate; MFG, monoferuloyl glycerol; DFG, diferuloyl glycerol; FGs, feruloyl glycerols; ILs, ionic liquids; [BMIM]PF6, 1-N-butyl-3-methylimidazolium hexafluorophosphate; [EMIM]TF2N, 1-ethyl-3-methylimidazolium bis (trifluorome
First Page Preview
Kinetics of enzymatic synthesis of monoferuloyl glycerol and diferuloyl glycerol by transesterification in [BMIM]PF6
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 97, 15 May 2015, Pages 25–31
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us