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Discerning the effects of photoinhibition and photoprotection on the rate of oxygen evolution in Arabidopsis leaves

Paper ID Volume ID Publish Year Pages File Format Full-Text
29654 44427 2015 7 PDF Available
Title
Discerning the effects of photoinhibition and photoprotection on the rate of oxygen evolution in Arabidopsis leaves
Abstract

•The relationship between qPd and ΦPSII of O2 evolution was tested.•It was linear for purely photoinhibitory conditions.•Use of qPd parameter allows to distinguish between NPQ and photoinhibition.•The new light tolerance method is validated by O2 evolution measurements.

Higher plants possess a set of interconnected processes to regulate light harvesting. Non-photochemical quenching of chlorophyll a fluorescence (NPQ) is the fastest process activated to protect the photosystem (PS) II from the absorption of excess light energy. However, damage of PSII reaction centers (RCIIs) is often inevitable, a phenomenon known as photoinhibition. Both NPQ and photoinhibition undermine PSII quantum yield (ΦPSII). Recently, we devised a fluorescence-based methodology that uses the coefficient of photochemical quenching measured in the dark following illumination (qPd) to assess the intactness of RCIIs. This procedure enables to express ΦPSII as a function (ƒ) of NPQ and qPd, ΦPSII = ƒ(NPQ,qPd), thus allowing to efficiently discern between the effects of protective NPQ and photoinhibition upon the efficiency of electron transport. In this study, we addressed the relationship between qPd and ΦPSII measured by photosynthetic oxygen evolution in intact leaves of Arabidopsis. We found a linear correlation between qPd and ΦPSII of oxygen evolution (as well as Fv/Fm). This relates to the fact that qPd reflects the onset of photoinhibition. These results further demonstrate the validity of the qPd parameter and underlying theory in quantitatively assessing PSII efficiency solely by using this effective and simple fluorescence technique.

Keywords
Φ, quantum yield; AL, actinic light; NPQ, non-photochemical quenching of chlorophyll fluorescence; pNPQ, protective non-photochemical quenching of chlorophyll fluorescence; PSII, photosystem II; qPd, photochemical quenching in the dark; RCII, PSII reactio
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Discerning the effects of photoinhibition and photoprotection on the rate of oxygen evolution in Arabidopsis leaves
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 152, Part B, November 2015, Pages 272–278
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us