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Aquaporin-deficient mutant of Synechocystis is sensitive to salt and high-light stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
29665 44427 2015 6 PDF Available
Title
Aquaporin-deficient mutant of Synechocystis is sensitive to salt and high-light stress
Abstract

•The AqpZ-null Synechocystis was used to study the responses to salt and light stress.•The AqpZ-deficient cells cannot regulate the cytoplasmic volume under salt stress.•PSII and PSI of the mutant are more sensitive to NaCl and to high light.•AqpZ participates in regulation of the PSI and PSII under salt and high-light stress.•AqpZ might be necessary for the repair of PSII and PSI after photodamage.

Cyanobacterial aquaporins play an important role in the regulation of various physiological functions: cell volume control, osmotic stress responses, gas exchange. We employed the AqpZ-deficient mutant of Synechocystis to study the role of aquaporins in responses to salt (NaCl) and high light stress. Electron microscopy and paramagnetic resonance revealed that AqpZ-deficient cells are unable to efficiently regulate the cytoplasmic volume under salt stress. Both photosystems (PSII and, especially, PSI) of these cells are more sensitive to NaCl and to high light. Thus, AqpZ of Synechocystis participates in regulation of the photosynthetic activity of PSI and PSII under salt and high-light stress. Our results demonstrate that AqpZ might be necessary for the repair of PSII and PSI after photodamage.

Keywords
BQ, 1,4-benzoquinone; Chl, chlorophyll; DCBQ, 2,6-dichloro-1,4-benzoquinone; DCIP, 2,6-dichloroindophenol; DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; EPR, electron paramagnetic resonance; MV, methyl viologen; PSI, photosystem I; PSII, photosystem IISy
First Page Preview
Aquaporin-deficient mutant of Synechocystis is sensitive to salt and high-light stress
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 152, Part B, November 2015, Pages 377–382
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering