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Synthesis of chloro-substituted analogs of Thiazole orange – Fluorophores for flow cytometric analyses

Paper ID Volume ID Publish Year Pages File Format Full-Text
29673 44429 2011 7 PDF Available
Title
Synthesis of chloro-substituted analogs of Thiazole orange – Fluorophores for flow cytometric analyses
Abstract

Synthesis, absorption and fluorescence properties of a series of asymmetrical monomethine cyanine dyes, chloro-containing analogs of Thiazole orange, are reported. Their staining ability was studied by flow cytometry. The saturating concentrations of each dye that gives a stable staining intensity have been determined. The ability of dyes B9, B11, B13 to stain live macrophages and apoptotic splenocytes was investigated. Positive signal in nucleus of adherent macrophages detected by fluorescent microscopy showed good specificity of B9, B11 and B13 dyes for DNA. In apoptotic assay cells positive for Annexin V were stained more brightly with the dyes B9, B11 and B13 than with propidium iodide. Despite that B13 showed high DNA selectivity it induces apoptosis of splenocytes and it is not suitable for detection of dead cells. The other synthesized chloro-containing analogs of Thiazole orange B9 and B11 can be successfully used for flow cytometric analyses of DNA content in live cells and for analyses of cell apoptosis.

► Synthesis of chloro-containing analogs of Thiazole orange has been reported. ► Spectral properties of the dyes in presence of dsDNA have been investigated. ► The ability of dyes to stain live or apoptotic cells were examined by flow cytometry. ► The dyes are suitable for detection of apoptosis at low concentrations (1 × 10−11 M).

Keywords
Thiazole orange analogs; DNA-binding dye; Apoptosis; Flow cytometry; Fluorescence
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Synthesis of chloro-substituted analogs of Thiazole orange – Fluorophores for flow cytometric analyses
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 103, Issue 3, 2 June 2011, Pages 215–221
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us