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Model based fed batch cultivation and elicitation for the overproduction of ajmalicine from hairy roots of Catharanthus roseus

Paper ID Volume ID Publish Year Pages File Format Full-Text
2968 145 2015 8 PDF Available
Title
Model based fed batch cultivation and elicitation for the overproduction of ajmalicine from hairy roots of Catharanthus roseus
Abstract

•Batch mathematical model was developed for ajmalicine production.•Kinetic model was extrapolated for fed batch cultivation and three strategies were experimentally implemented.•A 2.5 fold increase in ajmalicine content with increasing feed fed batch was obtained.•Combination of fed batch and elicitation led to 4 fold increase in ajmalicine concentration.

Batch cultivation of Catharanthus roseus hairy roots was attempted in a statistically optimized nutrient medium. This resulted in a biomass growth of 5.12 ± 0.46 g/l and ajmalicine content of 24.9 ± 1.2 mg/l on day 30 beyond which the product concentration declined. Using the observed batch kinetics data a mathematical model was developed which could describe the experimental observations to the extent of 99% accuracy as demonstrated by the statistical validity test. The model was thereafter extrapolated to fed-batch cultivation to identify suitable nutrient feeding strategies (by off-line simulations) for the overproduction of ajmalicine. Eventually the experimental implementation of three such feeding strategies and comparison with the model predictions further reinforced the efficacy of the mathematical model under dynamic fed batch conditions. A 60% increase in ajmalicine overall productivity and a 2.5 fold increase in volumetric yield was obtained by the “increasing feed rate strategy” as opposed to batch cultivation. To further improve the alkaloid accumulation a combination of statistically optimized mixture of elicitor(s) (jasmonic acid, methyl jasmonate and KCl) was added after the termination of model based increasing fed batch cultivation which resulted in a significantly high ajmalicine concentration of 123.2 ± 8.63 mg/l (a 4 fold increase compared to batch). This is the first report of such a strategy for overproduction of ajmalicine in rather complex hairy root cultivation.

Keywords
Ajmalicine; Modelling; Fed batch culture; Kinetic parameters; Elicitation; Plant cell culture
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Model based fed batch cultivation and elicitation for the overproduction of ajmalicine from hairy roots of Catharanthus roseus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 97, 15 May 2015, Pages 73–80
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us