fulltext.study @t Gmail

A comparative study of cytotoxicity and interaction with DNA/protein of five transition metal complexes with Schiff base ligands

Paper ID Volume ID Publish Year Pages File Format Full-Text
29710 44431 2015 10 PDF Available
Title
A comparative study of cytotoxicity and interaction with DNA/protein of five transition metal complexes with Schiff base ligands
Abstract

•Five transition metal complexes were synthesized and characterized.•In vitro cytotoxicities of all compounds were tested against five cancer cell lines.•DNA/BSA interactions of all compounds were studied.

Five transition metal complexes NiL12 (1), CuL12 (2), ZnL12 (3), [MnL12(N3)]n·nCH2Cl2 (4), CuL22 (5) {HL1 = 3-{[2-(2-hydroxy-ethoxy)-ethylimino]-methyl}-naphthalen-2-ol, HL2 = 2-{[2-(2-hydroxy-ethoxy)-ethylimino]-methyl}-phenol} have been synthesized and fully characterized. In all of the complexes, the ligands coordinated to the metal ion in a negative fashion via O and N donor atoms. The X-ray structures of nickel complex 1 and copper complexes 2 and 5 are four-coordinated monomers and show slightly distorted square-planar geometry in the vicinity of the central metal atom. Zinc complex 3 exhibits a four-coordinated tetrahedral structure. Differently, manganese complex 4 reveals a six-coordinated octahedral structure, one-dimensional chain is linked by azide in the end-to-end mode. In vitro cytotoxicity of these complexes to various tumor cell lines was assayed by the MTT method. The results showed that most of these metal-Schiff base complexes exhibited enhanced cytotoxicity than Schiff base ligands, which clearly implied a positive synergistic effect. Moreover, these complexes appeared to be selectively active against certain cell lines. The interactions of these metal complexes with CT-DNA were investigated by UV–vis, fluorescence and CD spectroscopy, the results indicated that these complexes are metallointercalators and can interact with CT-DNA. The study of interaction between complexes and BSA indicated that all of the complexes could quench the intrinsic fluorescence of BSA in a static quenching process.

First Page Preview
A comparative study of cytotoxicity and interaction with DNA/protein of five transition metal complexes with Schiff base ligands
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 148, July 2015, Pages 232–241
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering