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Probing the binding interaction of human serum albumin with three bioactive constituents of Eriobotrta japonica leaves: Spectroscopic and molecular modeling approaches

Paper ID Volume ID Publish Year Pages File Format Full-Text
29715 44431 2015 9 PDF Available
Title
Probing the binding interaction of human serum albumin with three bioactive constituents of Eriobotrta japonica leaves: Spectroscopic and molecular modeling approaches
Abstract

•This study was finished by adopting molecular simulation and experimental verification.•DS3.1 was used to analyze the binding ability and predict the possible binding sites.•The local conformation of HSA was changed at different levels by three drugs.

Corosolic acid (CRA), maslinic acid (MA), and tormentic acid (TA) are three kind of bioactive constituents of Eriobotrta japonica leaves. In this study, plasma protein binding model prediction suggested that the binding ability to HSA was CRA > MA > TA. Furthermore, fluorescence spectroscopy confirmed this prediction. The results from emission and time resolved fluorescence studies revealed that the emission quenching of HSA with CRA, MA, and TA were all initiated by static quenching mechanism. From molecular docking results and site marker competitive experimental results it was possible to make good estimates about CRA, MA, and TA mainly bound to subdomain IIA of HSA. 3D fluorescence, FT-IR and CD spectra indicated that the local conformation of HSA molecules was affected by the presence of CRA, MA, and TA, but at different extents.

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Keywords
Human serum albumin; Corosolic acid; Maslinic acid; Tormentic acid; Molecular modeling
First Page Preview
Probing the binding interaction of human serum albumin with three bioactive constituents of Eriobotrta japonica leaves: Spectroscopic and molecular modeling approaches
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 148, July 2015, Pages 268–276
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering