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Glycyrrhizic acid (GA) inhibits reactive oxygen Species mediated photodamage by blocking ER stress and MAPK pathway in UV-B irradiated human skin fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
29724 44431 2015 7 PDF Available
Title
Glycyrrhizic acid (GA) inhibits reactive oxygen Species mediated photodamage by blocking ER stress and MAPK pathway in UV-B irradiated human skin fibroblasts
Abstract

•UVB potently induced oxidative stress, ER stress and MAPK activation in human skin fibroblasts.•Glycyrrhizic acid (GA) inhibited reactive oxygen species mediated photodamage in Hs68 cells.•GA blocked UVB induced activation of ER stress and MAPKs.•GA prevented loss of mitochondrial membrane potential and apoptosis in UVB treated Hs68 cells.•GA offers potent anti photodamage effect and can be exploited for cosmetic or therapeutic purpose.

BackgroundPreviously we have reported that generation of reactive oxygen species is the prime event responsible for calcium mediated activation of PERK-eIF2α-CHOP pathway and apoptosis in UV-B irradiated human skin fibroblasts (Hs68). We have also reported that glycyrrhizic acid (GA) mediates potent photoprotective activity against UV-B – irradiation-induced photodamage in human skin fibroblast.ObjectiveIn the present study, we aimed to investigate the role of GA in preventing oxidative stress mediated unfolded protein response (UPR) and mitogen activated protein kinases (MAPK) pathway.MethodsHuman skin fibroblast (Hs68) cells were exposed to UV-B radiations in lab conditions. Different parameters of UVB induced cellular and molecular changes were analysed using western-blotting, microscopy and flow cytometry.ResultsOur results show that GA has strong photoprotective action against UV-B induced cellular damage. It was observed that: (a) Oxidative disturbances and intracellular Ca2+ imbalance induced by UV-B irradiation was significantly restored by GA treatment; (b) activation of PERK-eIF2α-CHOP and MAPK pathway induced by UV-B was significantly blocked by GA; (c) Loss of mitochondrial membrane potential and apoptosis induced by UV-B were reduced by GA treatment.ConclusionBased on the above findings we conclude GA has a highly significant ROS quenching activity, thereby blocking the cascade of events including release of calcium from ER and subsequent ER stress, MAPK pathway and cellular demise. GA offers highly potent anti photodamage effect and can be exploited for cosmetic or therapeutic purposes.

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Keywords
GA, glycyrrhizic acid; MAPK, mitogen activated protein kinases; UPR, unfolded protein response; UV-B, ultra violet-B; ER, endoplasmic reticulum; PERK, RNA dependent protein kinase like ER kinase; PARP1, poly ADP-ribose polymerase; ROS, reactive oxygen spe
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Glycyrrhizic acid (GA) inhibits reactive oxygen Species mediated photodamage by blocking ER stress and MAPK pathway in UV-B irradiated human skin fibroblasts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 148, July 2015, Pages 351–357
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us