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Targeted nanoparticles for simultaneous delivery of chemotherapeutic and hyperthermia agents – An in vitro study ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
29764 44436 2014 10 PDF Available
Title
Targeted nanoparticles for simultaneous delivery of chemotherapeutic and hyperthermia agents – An in vitro study ☆
Abstract

•Indocyanine green and doxorubicin were entrapped into PLGA nanoparticles (IDNPs).•IDNPs were functionalized with antibodies for Human Epithelial Receptor-2 (AIDNPs).•We investigated AIDNPs for cancer targeted chemo-photothermal therapy in vitro.•AIDNPs showed selective chemo-phototherapy effects upon NIR-laser irradiation.

The purpose of this study was to prepare targeted Poly lactide-co-glycolide (PLGA) nanoparticles with simultaneous entrapment of indocyanine green (ICG) and doxorubicin (DOX) by surface decorating them with tumor specific monoclonal antibodies in order to achieve simultaneous therapy and imaging. ICG was chosen as an imaging and hyperthermia agent and DOX was used as a chemotherapeutic agent. ICG and DOX were incorporated into PLGA nanoparticles using the oil-in-water emulsion solvent evaporation technique. These nanoparticles were further surface decorated with antibodies against Human Epithelial Receptor-2 (HER-2) using carbodiimide chemistry. The uptake of antibody conjugated ICG-DOX-PLGA nanoparticles (AIDNP) was enhanced in SKOV-3 (HER-2 overexpressing cell lines) compared to their non-conjugated counterparts (ICG–DOX–PLGA nanoparticles (IDNP)). The uptake of antibody conjugated ICG–DOX–PLGA nanoparticles, however, was similar in MES-SA and MES-SA/Dx5 cancer cells (HER-2 negative cell lines), which were used as negative controls. The cytotoxicity results after laser treatment (808 nm, 6.7 W/cm2) showed an enhanced toxicity in treatment of SKOV-3. The negative controls exhibited comparable cytotoxicity with or without exposure to the laser. Thus, this study showed that these antibody conjugated ICG–DOX–PLGA nanoparticles have potential for combinatorial chemotherapy and hyperthermia.

Keywords
Doxorubicin; Drug delivery; Hyperthermia; Image-guided therapy; Indocyanine green; Molecular imaging; Theranostics
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Targeted nanoparticles for simultaneous delivery of chemotherapeutic and hyperthermia agents – An in vitro study ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 136, 5 July 2014, Pages 81–90
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us