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Effect of blue light emitting diodes on melanoma cells: Involvement of apoptotic signaling

Paper ID Volume ID Publish Year Pages File Format Full-Text
29798 44440 2015 7 PDF Available
Title
Effect of blue light emitting diodes on melanoma cells: Involvement of apoptotic signaling
Abstract

•Irradiation with blue LED was observed to reduce cell viability and to induce apoptotic cell death.•Mitochondrial membrane potential increased, and mitochondria-related apoptotic proteins were observed.•Phosphorylation of p53 increased at earlier times under blue LED irradiation.•Early growth rate of the melanoma cells was inhibited under blue LED irradiation.

The present study was undertaken to examine whether blue LED irradiation induces cellular apoptosis in B16-F10 cells and whether it blocks the early growth of melanoma cells in mice. Irradiation with blue LED was observed to reduce cell viability and to induce apoptotic cell death, as accompanied by exposure of phosphatidylserine on the plasma outside membrane and an accumulation of a sub-G1 population. Furthermore, the mitochondrial membrane potential increased, and mitochondria-related apoptotic proteins (cytochrome c, caspase 3, and PARP) were observed. In addition, the level of intracellular superoxide anion (O2−) gradually increased. Interestingly the phosphorylation of p53 increased at earlier times under blue LED irradiation, but reduced after exposure for a longer time. Additionally, the thickness of the mice footpad injected with B16-F10 cells decreased significantly until the 9th day of blue LED irradiation, indicating the inhibition of the early growth rate of the melanoma cells. Our data demonstrate that blue LED irradiation induces apoptotic cell death by activating the mitochondria-mediated pathway and reduces the early growth rate of melanoma cells. Further studies are needed to elucidate the precise mechanism of blue LED in melanoma cells.

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Effect of blue light emitting diodes on melanoma cells: Involvement of apoptotic signaling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 142, January 2015, Pages 197–203
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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