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Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Paper ID Volume ID Publish Year Pages File Format Full-Text
2981 146 2015 6 PDF Available
Title
Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system
Abstract

•We investigated l-xylulose production by E. coli harboring l-arabinitol dehydrogenase.•We optimized conditions for l-xylulose production by recombinant E. coli whole cells.•Coupling with NADH oxidase enhanced l-xylulose production by LAD up to 96% efficiency.•Immobilized whole cells maintain 65% of the yield after 7 cycles of successive re-use.

The biotransformation of l-arabinitol into l-xylulose was investigated using whole-cell biocatalysts. Efficient production of l-xylulose was accomplished using Escherichia coli that expressed l-arabinitol dehydrogenase (LAD). The production yield was enhanced by coupling LAD with a cofactor-regenerating NADH oxidase in an E. coli whole-cell biocatalyst system. Factors affecting the production of l-xylulose by this whole-cell biocatalyst system include reaction pH, maximal cell loading, and cofactor regeneration. Under optimized conditions, the conversion of l-arabinitol into l-xylulose was achieved with above 96% efficiency, a rate much higher than that reported in previous studies. Furthermore, the whole-cell system could be immobilized on calcium alginate; the immobilized cells showed good operational stability, retaining their relative productivity at 65% after 7 cycles of successive re-use.

Keywords
Biocatalysis; Cell-free extract; Cofactor regeneration; Enzyme-coupled system; Immobilization; l-xylulose
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Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 96, 15 April 2015, Pages 23–28
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us