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Binding of hydroxylated single-walled carbon nanotubes to two hemoproteins, hemoglobin and myoglobin

Paper ID Volume ID Publish Year Pages File Format Full-Text
29835 44444 2014 10 PDF Available
Title
Binding of hydroxylated single-walled carbon nanotubes to two hemoproteins, hemoglobin and myoglobin
Abstract

•This paper attests that HO-SWCNTs exhibited significant bindings to Hb and Mb.•Multi-noncovalent interactions stabled the HO-SWCNTs–Hb/Mb complex.•The secondary structures of Hb and Mb were destroyed by HO-SWCNTs.•Molecular calculation showed that there were the preferable binding sites of HO-SWCNTs on Hb and Mb.

Herein, we studied the binding interactions between hydroxylated single-walled carbon nanotubes and hemoglobin and myoglobin by the use of multi-spectral techniques and molecular modeling. The ultraviolet–vis absorbance and circular dichroism spectral results indicated that the binding interactions existed between hydroxylated single-walled carbon nanotubes and hemoglobin/myoglobin. These binding interactions partially affected the soret/heme bands of hemoglobin and myoglobin. The secondary structures of hemoproteins were partially destroyed by hydroxylated single-walled carbon nanotubes. Fluorescence studies suggested that the complexes formed between hydroxylated single-walled carbon nanotubes and hemoglobin/myoglobin by hydrogen bonding, hydrophobic, and π–π stacking interactions. In addition, molecular modeling analysis well supported the experimental results.

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Keywords
Carbon nanotubes; Hemoglobin; Myoglobin; Conformation change; Binding mode
First Page Preview
Binding of hydroxylated single-walled carbon nanotubes to two hemoproteins, hemoglobin and myoglobin
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 141, December 2014, Pages 26–35
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering