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Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
2984 146 2015 9 PDF Available
Title
Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture
Abstract

•A KGM-based microcarrier was prepared for anchorage-dependent animal cell culture.•Five cell lines adhered well and grew to high cell density on KGM microcarriers.•Vero cells achieved more than 2 × 106 cells/ml on KGM microcarriers in spinner culture.•Cell density and rabies virus titer were comparable in KGM and Cytodex 1 culture.•The KGM microcarrier could be a promising candidate for various cell cultures.

A new type of microcarrier was prepared for anchorage-dependent animal cell culture. Similar to the commercial product Cytodex 1, the newcomer is also spherical in micro-size range with ionic group DEAE, but the matrix is konjac glucomannan (KGM) from a natural plant. The optimal anion exchange capacity (AEC) was found to be 2.1 to 2.4 mmol/g dry microspheres. Five cell lines were cultured in static mode with the KGM microcarriers, including Vero, CHO-K1, MDCK, Wish and L929 cells. These cells adhered well and grew to high cell density comparable with or even better than those did on Cytodex 1. A further comparison was made with Vero cell culture in spinner mode. It was found that Vero cells achieved more than 2 × 106 cells/ml, and showed faster cell adhesion and higher specific growth rate on the KGM microcarriers than on Cytodex 1. In 5 L bioreactor perfusion culture, Vero cells grew above 7.0 × 106 cells/ml and the rabies virus titer was about 7.7 lgLD50/ml, compared to Cytodex 1 in the same culture condition. The results suggest that the new type of microcarrier could be a promising candidate for various cell cultures.

Keywords
Konjac glucomannan; Microcarriers; Animal cell culture; Anion exchange capacity; Large-scale cultivation; Glucose
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Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 96, 15 April 2015, Pages 46–54
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us