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Green synthesized silver nanoparticles using Nelumbonucifera root extract for efficient protein binding, antioxidant and cytotoxicity activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
29843 44444 2014 6 PDF Available
Title
Green synthesized silver nanoparticles using Nelumbonucifera root extract for efficient protein binding, antioxidant and cytotoxicity activities
Abstract

•AgNPs were developed using the root extract of Nelumbo nucifera.•Green synthesis is a simple, rapid, clean, inexpensive and eco-friendly.•The interaction of green synthesized AgNPs with Bovine Serum Albumin (BSA).•Fluorescence quenching, synchronous and RLS influences the efficient binding between BSA–AgNPs.•AgNPs showed moderate antioxidant and cytotoxicity activity against HeLa cell lines.

Silver nanoparticles (AgNPs) with a mean particle size of ∼16.7 nm were synthesized using an eco-friendly reducing material, aqueous Nelumbo nucifera root extract. Rapid reduction resulted in the formation of polydispersed nanoparticles. The formation of AgNPs was characterized by surface plasmon resonance, which was determined by ultraviolet–visible (UV–Vis) spectroscopy (band at 412 nm), Fourier transform infrared spectroscopy, scanning electron microscopy-energy dispersive X-ray spectroscopy, transmission electron microscopy and X-ray diffraction. The interaction of the green synthesized AgNPs with Bovine Serum Albumin (BSA) at various temperatures was investigated. Fluorescence quenching, synchronous and resonance light scattering spectroscopy along with UV–Vis absorption studies revealed the efficient binding between BSA and the AgNPs. In addition, the AgNPs exhibited moderate antioxidant and cytotoxicity activities against HeLa cell lines.

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Green synthesized silver nanoparticles using Nelumbonucifera root extract for efficient protein binding, antioxidant and cytotoxicity activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 141, December 2014, Pages 100–105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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