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Advances and challenges in label-free nonlinear optical imaging using two-photon excitation fluorescence and second harmonic generation for cancer research

Paper ID Volume ID Publish Year Pages File Format Full-Text
29847 44444 2014 11 PDF Available
Title
Advances and challenges in label-free nonlinear optical imaging using two-photon excitation fluorescence and second harmonic generation for cancer research
Abstract

•Label-free nonlinear optical imaging (NLOI) is a promising tool for cancer diagnosis.•Label-free NLOI can detect microscopic changes in cancerous tissues non-invasively.•Label-free NLOI can monitor metabolic and collagen related changes during carcinogenesis.•Clinical translation of this modality requires optimising biosafety issues and improving deeper tissue imaging.•Other challenges include miniaturising NLOI setups for better access to internal organs.

Nonlinear optical imaging (NLOI) has emerged to be a promising tool for bio-medical imaging in recent times. Among the various applications of NLOI, its utility is the most significant in the field of pre-clinical and clinical cancer research. This review begins by briefly covering the core principles involved in NLOI, such as two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG). Subsequently, there is a short description on the various cellular components that contribute to endogenous optical fluorescence. Later on the review deals with its main theme – the challenges faced during label-free NLO imaging in translational cancer research. While this review addresses the accomplishment of various label-free NLOI based studies in cancer diagnostics, it also touches upon the limitations of the mentioned studies. In addition, areas in cancer research that need to be further investigated by label-free NLOI are discussed in a latter segment. The review eventually concludes on the note that label-free NLOI has and will continue to contribute richly in translational cancer research, to eventually provide a very reliable, yet minimally invasive cancer diagnostic tool for the patient.

Keywords
Cancer diagnostics; Nonlinear optical imaging; Two-photon excitation fluorescence; Second harmonic generation; Label-free; Endogenous optical fluorescence
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 141, December 2014, Pages 128–138
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us